A Vanderbilt Laboratory has an immediate opening for a Term Research Assistant II. The focus of the laboratory is on identifying the mechanisms by which the MAPK signal transduction pathway contributes to normal homeostasis and to disease. The candidate must have demonstrated experience in sterile cell culture and imaging and rodent handling. The ideal candidate will also have experience in immunofluorescence imaging as well as biochemistry and molecular biology techniques.
Conduct tissue culture studies using primary and transformed tissue and/or cells.
Perform rodent husbandry, genotype analysis, surgery and drug treatment
Flow cytometry analysis
Design and create expression vectors including plasmid and lentivirus
Write experimental protocols and accurately document all laboratory activities
Maintain chemical and biological inventory and safety documentation
Train or assist in training of new students and staff
Report Data to the PI in written, graphical, and oral communications.
Minimum Education: BA/BS in biology, chemistry or physics
Proficient with sterile tissue culture and experience in imaging
Willingness to work with primary human tissue
Willingness to work with rodents in a laboratory setting
Experience with animal handling, cloning, and Western blot
Basis skills in Microsoft Word, Excel and PowerPoint required with some coding experience in R highly desirable
Strong written and verbal communication skills
Demonstrated ability to work with others in a positive and productive manner
Individuals should be self- starters, have attention to detail, able to multi-task and capable of self-management
Willingness to perform some weekend and after hours work with possible overtime or adjusted weekday hours
Vanderbilt University Medical Center is home to Vanderbilt University Hospital, The Monroe Carell Jr. Children’s Hospital at Vanderbilt, the Vanderbilt Psychiatric Hospital and the Vanderbilt Stallworth Rehabilitation Hospital. These hospitals experienced more than 61,000 inpatient admissions during fiscal year 2015. Vanderbilt’s adult and pediatric clinics treated nearly 2 million patients during this same period.Vanderbilt University Hospital and the Monroe Carell Jr. Children’s Hospital at Vanderbilt are recognized again this year by U.S. News & World Report’s Best Hospitals as among the nation’s best with 18 nationally ranked specialties. Vanderbilt University Medical Center is world renowned because of the innovation, work ethic and collegiality of its employees. From our health care advances to our compassionate care, Vanderbilt owes its accomplishments and reputation to staff and faculty who bring skill and drive and innovation to the medical center day after day. World-leading academic departments and comprehensive centers of excellence pursue scientific discoveries and transformational educational and clinical advances across the entire spectrum of health and disease.As th...e largest employer in middle Tennessee, we welcome those who are interested in ongoing development in a caring, culturally sensitive and professional atmosphere. Most of us spend so much of our lives at work, we want to be part of maintaining a workplace in which people support one another and encourage reaching for excellence. Many high-achieving employees stay at Vanderbilt because of the professional growth they experience and because of their appreciation of Vanderbilt’s benefits, public events and discussions, athletic opportunities, beautiful setting and, above all, sense of community and purpose.Vanderbilt and its employees share a set of mutual expectations that have been created with productivity, legality, fairness and safety always in mind. We believe that our investment in training and compensating employees multiplies in value when we enable individuals to deliver their best performance for the benefit of us all.